Fig. 1.

Identification of the TU6-vgb⁺ transformant. a The vgb-expressing cassette from the plasmid pNOM102-VHb. TF and TR indicate the two primers used for PCR verification; b identification of the putative vgb transformant with PCR analysis. PCR products with the expected size (1.9 kb) are indicated by an arrow. Genomic DNA from the parental T. reesei strain TU-6 (lane 1), the transformant (lane 2) and vector DNA from pNOM102-VHb (lane 3) were used as DNA templates; c Western-blot analysis of the intracellular protein extracts from T. reesei after 48 h of growth in MM-glucose. Equal amounts of total protein were loaded in lanes 1–2. A band with the expected size (16 kDa) are indicated by an arrow. Lane 1 represents the parent strain TU-6 and lane 2 represents TU6-vgb⁺