Table 1.

Production media, cultivation conditions, CMC, yields, structures and references of the biodemulsifiers tested

Microorganisms	Production medium	Cultivation condition	CMC (mg/l)	Biosurfactant structure	Biosurfactant yield (g/l)	Reference
C. sphaerica	Distilled water supplemented with 9% soybean oil refinery residue + 9% corn steep liquor	28 °C and 200 rpm for 144 h	250	Glycolipid	9.0	Luna et al. (2013)
C. lipolytica (a)	Mineral medium supplemented with 6% soybean oil refinery residue + 1% glutamic acid	28 °C and 150 rpm for 72 h	300	Lipopeptide	8.0	Rufino et al. (2014)
C. lipolytica (b)	Distilled water supplemented with 5% animal fat + 2.5% corn steep liquor	28 °C and 200 rpm for 144 h	800	Glycolipid	2.2	Santos et al. (2013, 2017)
C. guilliermondii	Distilled water supplemented with 2.5% molasses + 4.0% corn steep liquor + 2.5% soybean oil refinery residue	28 °C and 200 rpm for 144 h	4200	Probably a glycolipid	2.1	Sarubbo et al. (2016)
P. cepacia	Mineral medium supplemented with 2% waste frying oil + 3% corn steep liquor	30 °C and 200 rpm for 144 h	156	Probably a glycolipid	5.2	Rocha e Silva et al. (2014)
Bacillus sp.	Mineral medium supplemented with 3% sugar cane molasses + 3% corn steep liquor	27 °C and 200 rpm for 120 h	5000	Probably a lipopeptide	10.5	Chaprão et al. (2015)
P. aeruginosa	Mineral medium supplemented with 3% glycerol + 0.6% sodium nitrate	28 °C and 200 rpm for 96 h	700	Glycolipid	8.0	Silva et al. (2010)