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. 2017 Nov 15;7:15655. doi: 10.1038/s41598-017-15546-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dietary-induced hypercholesterolemia increases cellular FoxP3 expression levels and Treg cell populations in spleen and mesenteric lymph nodes. FoxP3 expression in mice fed cholesterol-free standard chow diet (SCD) or 0.15% cholesterol-containing Western diet (WD) for 4 weeks. (A) Representative contour plots of live CD4+ T cells (SCD black lines; WD red lines) as well as histograms (SCD in white; WD in gray) and bar graphs of live CD4+ CD25+ T cells from spleen (upper panel) and mesenteric lymph nodes (mLN) (lower panel) derived from SCD (n = 4) or WD (n = 5) fed mice. (B) FoxP3+ Treg cell populations among live CD4+ T cells as in (A). Data for spleen (n = 21) and mLN (n = 10) from six and four independent experiments are shown, respectively. (C) Representative contour plots (SCD black lines; WD red lines) as well as histograms and bar graphs (SCD in white; WD in gray) of FoxP3 reporter mice (DEREG) gated on live CD4+ T cells (upper panel) or live CD4+ GFP+ Treg cells (lower panel) derived from spleens of SCD (n = 5) or WD (n = 3) fed mice. One representative experiment out of three is shown. (D) FoxP3 immunoblots among splenocytes (upper panel) and mesenteric lymph nodes (mLN) (lower panel) and quantification of relative band intensities of SCD or WD fed mice. All data are expressed as mean ± SEM; two-tailed Mann Whitney U test was performed for statistical analysis; *p < 0.05; **p < 0.01.