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. 2017 Nov 15;7:15655. doi: 10.1038/s41598-017-15546-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hypercholesterolemia increases population size and proliferation of FoxP3+ T cells in the thymus. Flow cytometry analysis of the thymus following 4 weeks of cholesterol-free standard chow diet (SCD) or 0.15% cholesterol-containing Western diet (WD). (A) Representative contour plots of live CD4+ thymocytes from SCD or WD treated mice and percentages of the CD4+ CD25+ FoxP3+ population are shown. Percentage of (B) CD4+ CD25+ FoxP3+ among CD4+ thymocytes in SCD (white, n = 6) or WD (gray, n = 7) fed mice and (C) Ki-67+ among CD4+ CD25+ FoxP3+ thymocytes in SCD (white, n = 3) or WD (gray, n = 4) fed mice. Values are expressed as mean ± SEM; ; two-tailed Mann Whitney U test was performed for statistical analysis; *p < 0.05, **p < 0.01.