Fig. 2.

Substrate activity of the human NUDIX hydrolases. a Activity of 18 human NUDIX hydrolases toward 52 substrates. Activity is represented in a heat map in which the absorbance at 630 nm normalized to untreated controls (this is, without BIP or PPase) is shown. The data represented correspond to the high enzyme concentration condition (200 nM); for the complete data set, see Supplementary Fig. 2d. b Hierarchical clustering heat map of the NUDIX hydrolases that displayed activity toward the corresponding substrates. Three distinct clusters appear containing MTH1, NUDT15, and NUDT18; NUDT5, NUDT9, NUDT12, and NUDT14; and NUDT2 and NUDT3. c NUDT2 and NUDT3 activity toward their corresponding substrates. d NUDT5, NUDT9, NUDT12, and NUDT14 activity toward their corresponding substrates. e MTH1, NUDT15, and NUDT18 activity toward their corresponding substrates. f Cluster co-assignment matrix comparing sequence similarity grouping and substrate activity clustering