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. 2017 Nov 15;7:15624. doi: 10.1038/s41598-017-15934-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of WCE on the mobilization and recruitment of myeloid cells in a nude mouse bearing PC-3 tumors. (a–c). The percentage of Gr-1⁺/CD11b⁺ myeloid cells and F4/80⁺/CD11b⁺ tumor-associated macrophages in primary tumors (a), peripheral blood (b) and lung (c) in tumor-bearing mice treated with WCE or vehicle as identified by FACS. (d,e) The percentage of MDSCs (d) and TAMs (d) in different tissues are shown as means ± SE (n = 3). (f) Fractions of Ly6G⁺/MMP9⁺ and Ly6G⁺/S100A8⁺ cells in primary PC-3 tumors (left) and in the lung (right) were calculated (n = 5). (g,h) The representative image of Ly6G⁺/ MMP9⁺ cells (g) and Ly6G⁺/S100A8⁺ cells (h) in primary PC-3 tumors and lungs were detected by immunofluorescent staining. Bar, 20 μm. All values are represented as means ± SEM. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 (t-test, two-tail).