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. 2017 Nov 15;8:1524. doi: 10.1038/s41467-017-01808-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — PLIC analysis of caspase-1 oligomerization in NLRP3 inflammasome-activated bone-marrow derived macrophages (BMDM). a Light microscopy images (x20) of non-activated BMDM and NLRP3 inflammasome-activated (LPS + ATP) BMDM. Scale bar 100 μM b Representative images of non-activated and NLRP3 inflammasome-activated BMDM, detected by PLIC, depicting median cells of the PLA bright detail intensity, and the PLA max pixel features. Cells are presented as (left to right): bright field (BF), staining of F4/80 (PE/Cy7), CD11b (PB), BF/PLA/F4/80/CD11b overlay (merged), PLA (FarRed), and side scatter (SSC). Scale bar 7 μM. c Quantitative analysis of caspase-1 oligomerization (presented as the percentage of PLA⁺ cells) in non-activated cells, cells at peak activation, and probes only control. Representative of four independent experiments with similar results, calculated from 20,000 cells (F4/80⁺CD11b⁺) per treatment (no activation/ NLRP3 inflammasome activation by LPS + ATP)