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. 2017 Nov 15;7:15650. doi: 10.1038/s41598-017-15834-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scn3a-encoded Na_V1.3 is specific to EC cells of mouse and human. (A) Expression of EC cell marker Tph-1 and voltage-gated sodium channel gene Scn3a by RNAseq in FACS-sorted Tph1-CFP EC cells from mouse small bowel. Red, TPH1-CFP; green, Na_V1.3; blue, DAPI. Scale bar, 10 µm; inset, 5 µm. Inset, Na_V1.3 is localized at the basal side of mouse EC cells. (B) Percentages of Na_V1.3+/5HT+ cells out of all EC (5HT+) cells in human (blue) and Na_V1.3+/CFP+ cells out of all EC cells (CFP+) in mouse (cyan) jejunum (left) and colon (right), as counted from immunohistochemistry data.