Table 1.
NaV channels in primary EC cells have similar properties.
| ECJ | ECC | |
|---|---|---|
| Preps (n) | 44 | 13 |
| Cells (n) | 125 | 18 |
| P(INa) (%) | 81.3 ± 4.0 | 64.1 ± 9.2 |
| C (pF) | 3.9 ± 1.0 | 2.1 ± 0.2* |
| IPEAK/C (pA/pF) | −63.4 ± 5.6 | −68.0 ± 11.5 |
| GMAX (pA/pF) | 1.57 ± 0.13 | 1.60 ± 0.19 |
| V1/2I (mV) | −48.2 ± 1.1 | −53.3 ± 3.8 |
| δVI | 7.1 ± 0.1 | 8.9 ± 0.9 |
| V1/2A (mV) | −23.4 ± 0.9 | −25.3 ± 2.2 |
| δVA | 6.7 ± 0.2 | 6.5 ± 0.3 |
| EREV (mV) | 39.0 ± 2.1 | 31.8 ± 4.3 |
| τA (ms) | 0.26 ± 0.02 | 0.23 ± 0.03 |
| τF (ms) | 1.00 ± 0.04 | 0.68 ± 0.04* |
| τS (ms) | 11.4 ± 0.6 | 11.2 ± 1.1 |
Parameters of Na+ current under control conditions in CFP+ enterochromaffin cell cultures from mouse small bowel (ECJ) or colon (ECC). Preps, preparations of EC cell primary cultures. Cells, patch clamped murine TPH1-CFP+ EC cells with measurable Na+ current. P(I Na), average proportion of EC cells with measurable Na+ current in any given EC preparation. C, cell capacitance. I PEAK /C, peak Na+ current normalized to cell capacitance. G MAX, maximum conductance. V 1/2I, voltage of half inactivation. δV I, slope of voltage dependence of inactivation. V 1/2A, voltage of half activation. δV A, slope of voltage dependence of activation. E REV, reversal potential of Na+ current. τ A, time constant of activation. τ F, time constant of fast inactivation. τ S, time constant of slow inactivation. Values shown for time constants are at 0 mV. *P < 0.05 ECC vs. ECJ by a non-parametric two-tailed t-test.