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. 2017 Nov 16;8:1534. doi: 10.1038/s41467-017-01199-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Negative feedback regulation of Fbxo4 by Fxr1. a Fbxo4 protein levels upon Fxr1 knockdown in 74A, 74B and SCC9 cells. b Fbxo4 mRNA levels upon Fxr1 knockdown in 74A, 74B and SCC9 cells. c Fbxo4 protein levels upon ectopic Fxr1 expression in U2OS and NIH3T3 cells. d Fbxo4 mRNA levels upon ectopic Fxr1 expression in HEK293T, U2OS and NIH3T3 cells. e Polysome profile in NIH3T3 cells with Fxr1 overexpression. f DNA gel shows the RT-PCR products from serial polysome fractions. g Quantitative distribution of Fbxo4 mRNA from (f). h Western blot from immunoprecipitation of Fxr1 in RIP analysis. i RIP analysis indicates Fxr1 interacts with Fbxo4 mRNA. TERC mRNA is used as positive control. All the data represent mean ± s.d. and were analysed by Student’s t test (n = 3). **p < 0.01