Fig. 7.

Identification of the AREs that control Fbxo4 translation by Fxr1. a Schematic illustration of AREs in 3′-UTR of Fbxo4 mRNA; AREs are indicated in blue. ARE prediction is performed using AREsite2: http://rna.tbi.univie.ac.at/AREsite2/welcome. b Deletions made based on the ARE distribution. FL: full-length; Del: Deletion. c Fxr1 knockdown promotes the luciferase activity-mediated by both 3′-UTRs of p21 and Fbxo4 mRNAs in 74B cells. d Fxr1 overexpression suppresses the luciferase activity-mediated by 3′-UTR of Fbxo4 mRNA in HEK293T cells. e ARE deletion rescues luciferase activity in HEK293T cells with Fxr1 overexpression. f ARE deletion reduces luciferase activity in 74B cells with Fxr1 knockdown. g The comparison of basal luciferase activity with full-length and deleting 3′-UTRs of Fbxo4 mRNA in 74B cells. h Proposed model summarises the regulation of Fxr1 by Fbxo4 and feedback regulation of Fbxo4 by Fxr1. All the data represent mean ± s.d. c, d were analysed by Student’s t test (n = 3). e–g were analysed by One-way ANOVA, followed by Fisher’s LSD as post hoc test (n = 3). **p < 0.01