Table 1.
Summary of crystallographic statistics.
| Data collection | CDC73(1–100) - native | CDC73(1–100) - derivatives | CDC73(1–111) - native |
|---|---|---|---|
| Space group | P2 1 | P21 | P212121 |
| Cell dimensions | |||
| A, b, c (Å) | 28.59, 53.50, 65.73 | 28.55, 53.52, 66.01 | 38.09, 43.68, 67.63 |
| α, β, γ (°) | 90.00, 95.66, 90.00 | 90.00, 95.73, 90.00 | 90.00, 90.00, 90.00 |
| Wavelength (Å) | 0.9782 | 0.9782 | 0.9782 |
| Resolution (Å) | 50–1.40 (1.42–1.40) | 50–1.40 (1.42–1.40) | 50–1.02 (1.05–1.02) |
| Rmerge (%) | 4.3 (18.1) | 11.5 (57.4) | 12.2 (35.9) |
| I/σI | 41.92 (10.0) | 72.38 (6.5) | 38.61 (4.9) |
| CC1/2 | 0.996 (0.986) | 0.989 (0.964) | 0.964 (0.891) |
| Completeness (%) | 98.9 (99.1) | 99.0 (99.5) | 99.0 (79.8) |
| Redundancy | 6.6 (6.3) | 25.3 (18.8) | 6.3 (5.8) |
| Wilson B factors (Å2) | 11.0 | 15.9 | 9.2 |
| Refinement | |||
| Resolution (Å) | 19.5–1.40 | 19.4–1.02 | |
| No. Reflections | 38451 | 57501 | |
| Rwork/Rfree (%) | 13.52/17.42 | 11.94/13.94 | |
| No. atoms | 1661 | 1740 | |
| No. of water | 348 | 284 | |
| Average B factors (Å2) | |||
| Protein | 15.37 | 13.18 | |
| H2O | 33.25 | 32.27 | |
| R.m.s deviations | |||
| Bond lengths (Å) | 0.012 | 0.011 | |
| Bond angles (°) | 1.361 | 1.516 | |
| Ramachandran plot (%) | |||
| Most Favorable | 100.0 | 100.0 | |
| Allowed | 0.0 | 0.0 | |
| Outliers | 0.0 | 0.0 | |