Figure 2.

CARMA1-deficiency only partially disrupts NKT cell development. (A) Thymic and splenic NKT cells from C57BL/6 (n = 9), Prkcq ^−/− (n = 9), Carma1 ^−/− (n = 5) and Prkcq ^−/−; Carma1 ^−/− (n = 3) mice were identified as CD3ε⁺tetramer⁺ cells within electronically gated CD8^lo thymocytes or B220^lo splenocytes. Numbers are % NKT cells among total leukocytes within each organ. (B) Absolute cell numbers were calculated from % NKT cell and total cell count. n, as in A. (C) Thymic and splenic CD4⁺ and CD8⁺ T cells from C57BL/6 (n = 3), Prkcq ^−/− (n = 3), Carma1 ^−/− (n = 3) and Prkcq ^−/−;Carma1 ^−/− (n = 3) mice were identified as CD4⁺ or CD8⁺ cells within electronically gated thymocytes or B220^lo CD3ε⁺ splenocytes. Numbers are % CD4⁺ or CD8⁺ T cells among total leukocytes. Data are mean ± sem (standard error of the mean) from 3 experiments; n, as in C. (D) Absolute cell numbers were calculated from % CD4, CD8, CD4⁺8⁺ DP or CD4⁻8⁻ DN and total cell count. n, as in C. Data are mean ± sem from 3 (A,B) or 2 (C,D) independent experiments. ns, not significant (P > 0.05); *P ≤ 0.05, **P ≤ 0.001.