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. 2017 Nov 15;7:15594. doi: 10.1038/s41598-017-15461-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fas-deficiency fails to rescue NKT cell development in IκBαΔN^tg. (A) Thymic, splenic and hepatic NKT cells from B6-wt/lpr (n = 2), B6-lpr/lpr (n = 2), B6-wt/lpr;IκBαΔN ^tg (n = 2) and B6-lpr/lpr;IκBαΔN ^tg (n = 2) mice were identified as CD3ε⁺tetramer⁺ cells within electronically gated CD8^lo thymocytes or B220^lo splenocytes. Numbers are % NKT cells among total leukocytes in each organ. (B) Absolute cell numbers were calculated from % NKT cell and total cell count. n, as in A. Data are mean ± sem from two independent experiments. ns, not significant (P > 0.05), *P ≤ 0.05.