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. 2017 Nov 15;7:15607. doi: 10.1038/s41598-017-15925-1

Figure 3.

Figure 3

GA reduces expression of cardiac-specific transcription factors in SHRs and angiotensin II-treated H9c2 cells. (A–D) Transcript levels of GATA4, GATA6, Nkx2-5, and SRF were evaluated by RT-PCR, for the three groups, at 24 weeks (n = 14~16 per group). The transcript levels have been normalized to 18S and presented as a relative value. **P < 0.01 and ***P < 0.001 versus WKY. # P < 0.05, ## P < 0.01, and ### P < 0.001 versus SHR. NS, not significant. (E,F), H9c2 cells were treated with either vehicle or GA (100 µM) for 9 h after exposure to angiotensin II (1 µM). The values are the mean ± SE of at least three independent experiments. (E) GATA4 and GATA6 mRNA were quantified using qRT-PCR. *P < 0.05 versus vehicle-treated cells. # P < 0.05 versus angiotensin II-treated cells. NS, not significant. (F) Nkx2-5 and SRF transcript levels in H9c2 cells. NS, not significant.