Fig. 2.

Reported HAT inhibitors exhibit common mechanisms of assay interference. a Several reported HAT inhibitors form adducts with GSH (denoted by apostrophe) in vitro as detected by UPLC-MS. Note for compound 3, the parent compound and the compound detected after treatment with CoA elute at different retention times (see inset). Data are representative results from at least two independent experiments. R, GSH. b Several reported HAT inhibitors are positive in an aggregation counter-screen. Compounds were tested for AmpC β-lactamase inhibition ± Triton X-100. Rottlerin, lidocaine, and fluconazole were included as positive control (PC) and negative control (NC1, NC2) compounds, respectively. Data are expressed as mean ± SD pooled from three or four independent experiments each performed with three technical replicates. See Table 1 for additional results and interpretations