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. 2017 Nov 15;7:15591. doi: 10.1038/s41598-017-15529-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maternal IE exposure increases Dnmts expression and DNA methylation in the thyroid of the adult male offspring. Thyroid lobes were obtained from the adult male offspring of control (C) or IE-exposed rat dams (5-HI). (A) Dnmt1 and Dnmt3 mRNA content were analyzed by Real-Time PCR and normalized to Rpl19 mRNA content (n = 10/group). (B) Total Dnmt1 and Dnmt3 protein content were analyzed in the thyroid through Western blotting, using Gapdh as loading control (n = 10/group). Representative western blots are shown in the left panel. (C) Thyroid DNA methylation was assessed through Imprint® Methylated DNA Quantification Kit. Results are expressed as means ± SEM as fold change, arbitrary units (AU) or percentage (%), respectively. *P < 0.05, **P < 0.01; ***P < 0.001 vs. C (Unpaired two tailed Student’s t-test).