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. 2017 Nov 15;7:15591. doi: 10.1038/s41598-017-15529-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maternal IE exposure induces post-translational modifications in the thyroid of the adult male offspring. Thyroid lobes were obtained from the adult male offspring of control (C) or IE-exposed rat dams (5-HI). Thereafter, the methylation status of the lysines 9 and 27 of histone H3 (A) and acetylation status of the histones H3 and H4 (B) were evaluated through Western blotting analysis, using Gapdh as loading control (n = 8/group). Representative western blots are shown in the left panels. (C) Thyroid Hat and Hdac mRNA expression were analyzed by Real-Time PCR and normalized to Rpl19 mRNA content (n = 10/group). (D) Thyroid HAT activity was measured with a colorimetric assay kit, while HDAC activity was assessed with a fluorimetric assay kit. Results are expressed as means ± SEM as arbitrary units (AU) (A-B), fold change (C) or percentage (%) (D),. *P < 0.05, **P < 0.01; ***P < 0.001 vs. C (Unpaired two tailed Student’s t-test).