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. 2017 Nov 15;7:15591. doi: 10.1038/s41598-017-15529-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of experimental protocol. Male and female Wistar rats were mated and after confirming the pregnancy through the presence of spermatozoa in the vaginal smear, the pregnant rats (GD1) were treated or not with water supplemented with 0.6 mg/L NaI during pregnancy and lactation periods. At postnatal day (PND) 1, the litters were culled to eight pups per dam and kept at this proportion until the end of the weaning. At PND21 the male rats were housed in cages and maintained with water and food ad libitum. At PND90, the adult male rats were anesthetized and euthanized by decapitation and tissues were collected for performing several molecular and biochemical analyses.