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. 2017 Nov 15;7:15614. doi: 10.1038/s41598-017-15057-6

Figure 3.

Figure 3

ATG3 interacts with negatively-charged phospholipid vesicles. Results from the flotation assay and quantification of the liposome-bound protein fraction of (A) 10 µM ATG3 or ATG3 K9D/K11D incubated with 3 mM LUVs (100 nm average size) (B) 10 μM ATG3 incubated with 3 mM SUVs (60 nm average size) (C) 10 μM ATG3 with LUV containing different anionic phospholipids. The gels show representative results. The data correspond to mean ± SEM (n = 3); **P = 0.001–0.01, ***P < 0.001. Full-length blots are presented in Supplementary Figure 3.