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. 2017 Nov 15;7:15609. doi: 10.1038/s41598-017-15411-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of experimental efficiency error to simulated behavior. (A) Shows FRET efficiency vs normalized fit residual for all of the pixels in each of the images in Fig. 7. (B) Comparison of experimental and simulated standard deviations showing strong agreement between the two. The experimental standard deviation estimates are only valid where there is a significant number of pixels to estimate it with so corresponding histograms are provided on the secondary axis.