. 2017 Nov 15;7:15609. doi: 10.1038/s41598-017-15411-8

Table 1.

Comparison of SensorFRET measurements (using both single and two photon excitation) to sRET and FLIM-FRET measurements for established FRET standards.

Method	FRET Efficiency
FRET Standard	CTV	C32V	C5V	VCV
SensorFRET(405/458 nm)	2.1 ± 5.6 (n = 14)	36.2 ± 2.9 (n = 23)	43.8 ± 6.5 (n = 24)	70.7 ± 3.1 (n = 62)
SensorFRET(850/920 nm)	0.8 ± 5.3 (n = 16)	30.7 ± 5.4 (n = 10)	47.5 ± 3.8 (n = 12)	NA
sRET	1.7 ± 7.0 (n = 12)^a	29.5 ± 8.0 (n = 60)b^b	41.5 ± 8.7 (n = 60)^b	69.7 ± 5.7 (n = 6)^a
FLIM-FRET	6.3 ± 2.6 (n = 30)^a	33.3 ± 4.5 (n = 10)^b	44.3 ± 1.7 (n = 10)^b	64.5 ± 3.2 (n = 20)^a

Values are reported as mean ± std (n = # of cells measured). Values denoted with ^awere obtained from Thaler et al.²⁰. Values denoted with ^bwere obtained from Koushick et al.²⁸.