Fig. 2.

Over-expression of NF-YC9 promotes, but down-regulation of NF-YC9 expression shows no effect on, stomatal response to ABA. a ABA inhibition of stomatal opening, and b ABA promotion of stomatal closure, in the wild-type Col-0, nf-yc9-1 knockdown mutant and the NF-YC9 overexpression transgenic lines OE1 and OE6. Mature rosette leaves from 3-week-old seedlings were used for the assays. Each value is the mean ± SE of three independent biological experiments, and different letters indicate significant differences at P < 0.05 (Duncan’s multiple range test) when comparing values within the same ABA concentration. c Photographs showing the stomatal aperture of the ABA-induced inhibition of stomatal opening in the different genotypes as described in (a). Bars represent 10 μm. d Photographs showing the stomatal aperture of the ABA-induced promotion of stomatal closure in the different genotypes as described in (b). Bars represent 10 μm