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. 2017 Nov 5;9(5):1501–1515. doi: 10.1016/j.stemcr.2017.10.003

Figure 7.

Figure 7

Quantitative Evaluation of RSA Detection in iPS-RPE Cell Transplanted Rejection Monkeys

We regularly collected the serum at 0, 1, 4, 6, 8, 12, and 16 weeks (W) after transplantation in a TLHM6 rejection model.

(A) Immunofluorescence analysis of 46a iPS-RPE cells incubated with the serum from the TLHM6 monkey. Nuclei were counterstained with DAPI (blue). PC, positive control staining. Scale bars, 50 μm.

(B) Quantification of immunofluorescent staining of RPE cells by monkey sera. The mean fluorescence intensity with confocal microscopy obtained by staining with the sera from TLHM6 monkeys is indicated (left panel). The right panel indicates the results of incubation with the sera from a DrpZ17 monkey (collected at 0, 1, 2, 4, 8, 12, and 24 weeks after transplantation) with MHC homozygote iPS-RPE cells (MHC haplotype-matched transplantation). ∗p < 0.05, compared with the control (0 weeks, before transplantation; open bar).

(C) Mean fluorescence intensity with confocal microscopy of staining of 46a iPS-RPE cells by the serum from the TLHM6 rejection monkey that was collected at 6 weeks after transplantation. Before incubation, the 46a iPS-RPE cells were pretreated with mouse IgG (1), anti-MHC-I antibodies (2), anti-MHC-II antibodies (3), or both antibodies (4). ∗∗p < 0.005, compared with the control (isotype control, open bar). Data are the means ± SEM of three independent experiments.