Figure 1.

Characterization of Sorted Cell Populations

(A) Protocols used for randomized, endoderm (DE), and mesoderm (ME) differentiation.

(B) Schematic overview of the experimental setup to purify the different populations.

(C) Representative flow-cytometric dot plot diagrams of HES3 cells in the undifferentiated state (ESC) or after 4 days of differentiation with the indicated protocols. Numbers represent the respective percentages of cells in the indicated quadrant. Depicted are CXCR4 and CD49e (upper panel) or EpCAM and NCAM (lower panel).

(D–F) Normalized expression of marker genes for pluripotency (NANOG, POU5f1, SOX2) (D), DE (FOXA2, SOX17, GSC) (E), and ME (PDGFRA, KDR, CD34) (F) scaled to hESC. Values are mean ± SEM, n = 4–7. ANOVA plus Bonferroni's post hoc test, ^∗p < 0.05, ^∗∗p < 0.01 compared with hESC. C⁻, CXCR4⁻ cells; C⁺, CXCR4⁺ cells; E⁺N⁻, EpCAM⁺/NCAM⁻ cells; E⁻N⁺, EpCAM⁻/NCAM⁺ cells.

See also Figures S1 and S2.