Figure 2.

Comparative Analysis of the miRNomes (HUES8) and Validation of Selected miRNAs in a Second hESC Cell Line (HES3)

(A) Comparison of the miRNA expression ratios of purified DE cells versus ME cells (y axis) or versus hESCs (x axis) using the HUES8 line.

(B) miRNA expression ratios of ME cells versus DE cells (y axis) or versus hESCs (x axis) (HUES8 cell line). (A) and (B) present results from pooled samples each comprising 4 independent experiments. Plotted are the 369 miRNAs expressed in all analyzed populations. The central quadrant represents the chosen 10-fold altered expression difference and the indicated numbers represent the miRNAs in the respective quadrant.

(C–F) Normalized expressions of the indicated miRNAs in the different populations (C and E) or during DE (D) or ME (F) differentiation, respectively. Values are mean ± SEM, n = 4–8. ANOVA plus Bonferroni's post hoc test, ^∗p < 0.05, ^∗∗p < 0.01 compared with all other populations in (C) and (E), and compared with day 0 in (D) and (F). C⁺, CXCR4⁺ cells; E⁻N⁺, EpCAM⁻/NCAM⁺ cells; C⁻, CXCR4⁻ cells; E⁺N⁻, EpCAM⁺/NCAM⁻ cells.

See also Figure S3.