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A strain disrupted in isocitrate lyase (ICL) and KGDH cannot grow on a minimal medium as it is blocked in all routes for citrate/isocitrate/2-ketoglutarate recycling. Overexpression of a 2-ketoglutarate-dependent dioxygenase (brown line) provides a sink for 2-ketoglutarate and thus enables growth, while catalyzing the conversion of a feedstock compound X to a desired compound Y.
