Table 1.

Various methods of immune complex dissociation and the minimum starting volume of canine serum or plasma needed to recover 100 μL for the heartworm antigen ELISA test

Reference	Method	Minimum volume of sample required to recover 100 μL for testing	Reagents added to the sample
Weil [5]	Heat + EDTA	100 μL	100 μL 0.1 M EDTA
IDEXX PetChek® HW antigen testa	Pepsin	50 μL	35 μL S1 (acid) + 35 μL S2 (base)
Pokriefka [13] HIV antigen test	Acidic pH	50 μL	35 μL S1 (acid) + 35 μL S2 (base)
Little [11]	Heat	600 μL	NA

^aThe sample is added to an enzyme-coated well of the microtiter plate from the PetChek® HW antigen test kit. Solutions S1 and S2 are reagents contained within the PetChek® HW antigen test kit and are added sequentially before transferring the mixture to the plate assay