Table 1.
Primers used in this study
| Primers | Sequences (5′–3′) | Target gene |
|---|---|---|
| creF | GTACTTTGGCCCTCGCTGAG | cre1 |
| creR | AGCAATCAGGTGCAGATATCAC | cre1 |
| creRUTr | CCAGACTGCATAAGGATTCCC | cre1 |
| cre1-2426UF | GCCAAGACTCAGCATAAA | cre1 |
| cre1-1059R | GCTAATGATGTCGGTAAGT | cre1 |
| cre1-497F | GCACTCCTACTCGTCCTT | cre1 |
| cre1-UF | CCTTCAATGGGGAGGTGG | Upstream region of cre1 |
| cre1-UR | TGGTGGGTGAGATAGACA | Upstream region of cre1 |
| cre1-DF | CAGCACAATACGACTCCG | Downstream region of cre1 |
| cre1-DR | TGCCGAATACCCTGAAAA | Downstream region of cre1 |
| DR-F | TTGTCCCAGCCCGAGGCATTAG | DR fragment |
| DR-R | AGCCGCTGGTCAATGTTATC | DR fragment |
| cre1-F1 | GGCGCCTGTGCCAGACTA | Δcre1::pyrG cassette |
| cre1-R1 | CAGCACAATACGACTCCG | Δcre1::pyrG cassette |
| pyrG-F | CGCCGTCGTGTCTCGTCT | A. niger pyrG |
| pyrG-R | ACAGACGGGTATAGGGGTA | A. niger pyrG |
| cbh1-1138UF | CCTTTGGCGTTTCCCTGATTC | Promotor of cbh1 |
| cbh1-F1 | AAAGCGTTCCGTCGCAGTAG | Promotor of cbh1 |
| cbh1-R1 | AGCACGAGCTGTGGCCAAGAAGG | Promotor of cbh1 |
| bglA-F1 | CAATAGTCAACCGCGGACTGCGCATCATGAGGTTCACTTTGATCGAGGCG | A. niger bglA |
| bglA-R1 | GTGGGTGGAGGGTGCTGGA | A. niger bglA |
| bg-F | GTATTACCCCTCCCCTTGG | A. niger bglA |
| bg-R | TCGTAGGCGATGTAGTGC | A. niger bglA |
| pyrG-UF1 | AATGCTCCGTAACACCCA | pyrG+DR fragment |
| pyrG-2425DR | ATCATCGTAACCGAGAATCCA | pyrG+DR fragment |
| cre1-probe-F | GGACTTGACACGGGCTAT | Probe |
| cre1-probe-R | AGCCATCTCGCAGTGTAT | Probe |