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. 2017 Nov 15;8:264. doi: 10.1186/s13287-017-0719-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cell morphology and cell labeling after 1 week of chondrogenic coculture. a Phase contrast images of IPFP mesenchymal stem cells (MSCs) (left), articular chondrocytes (ACs) (middle), and coculture of MSCs and ACs (right) in monolayer. The green arrow points to ACs, and the red arrow points to MSCs in coculture. b Macroscopic observation of MSCs (left), ACs (middle), and coculture of MSCs and ACs (right) in pellets. Arrows point to cell sediments after 1 week of culture; inserts illustrate the size of the pellets. c MSCs were labeled with DiI (red) and ACs were labeled with Dio (green) in monolayer (left) and pellet (middle) coculture. After 1 week of coculture, the labeled cells were counted, and the ratio of AC number to MSC number was calculated (right). Data are presented as mean ± SD. *P < 0.05