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. 2017 Nov 15;8:264. doi: 10.1186/s13287-017-0719-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Effects of chitosan/hyaluronic acid (CS/HA) NP exposure on IPFP mesenchymal stem cell (MSC) and articular chondrocyte (AC) cocultures. a Effect of the weight ratio of CS to HA on the size and zeta potential of NPs. Insert: TEM micrograph of CS/HA NPs. b Fluorescent images of cells exposed to FITC-conjugated CS/HA NPs for 1 h. c Morphology, hematoxylin and eosin (H&E), and Alcian blue staining of monolayer (top) and pellet (bottom) culture of MSCs + ACs + NPs. d Immunofluorescence assay of monolayer (top) and pellet (bottom) culture of MSCs + ACs + NPs. Col collagen, NC negative control