Fig. 1.

The effect of active kinase and dominant negative enzymes alone or in combination. ARPE-19 cells were transfected individually with AurA, AurAKD (kinase dead dominant negative), Nek2 and Nek2KD (kinase dead dominant negative), AurKD/Nek2, AurA/Nek2KD. Cilia number (a and b) and length (c and d) were measured for the AurA single and cotransfectants (a and c) and the Nek2 single and double transfectants (b and d). Measurements were made when cells were serum starved for 48 h (assembling cilia, left panels) and refed serum for 24 h (disassembling cilia, right panels). 300 cells were counted. Significant differences are indicated by like symbols; p ≤ 0.05. Empty vector is significantly different from all other transfectants when cilia are assembling. The means for cilia number and length ± SEM are shown in Table 1