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. 2017 Nov 15;18:33. doi: 10.1186/s12860-017-0149-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Nek2 and AurA form a complex in ARPE-19 cells. (a) Cells were cotransfected with plasmids expressing AurA and Nek2 and cell lysates immunoprecipitated with either AurA antibodies (lane 1), Nek2 antibodies (lane 2), or no antibody (land 3) and probed for either AurA or Nek2. (b) Untransfected ARPE-19 cell lysates were immunoprecipitated with either AurA (lane 1), Nek2 (lane 2), or no antibody and probed for either AurA or Nek2. Each immunoprecipitate was blotted for the immunoprecipitating antibody to verify immunoprecipitation