Fig. 5.

miR-200b decreases the expression of CFTR mRNA during normoxia and hypoxia. a Calu3 and 16HBE14o- cells were transfected with miR-200b mimic or antagomir and the mRNA levels were monitored in qRT-PCR experiments in normoxic conditions and after 12 h of hypoxia. CFTR mRNA levels from 2 independent experiments (n = 8) are normalized to 18S rRNA levels and expressed as a fold change over the transfection control. b The corresponding changes in CFTR protein levels of were detected with SDS-PAGE and Western Blot analyses and normalized to the β-actin levels. Two individual samples (3 μg of total protein per lane) were tested for each treatment and the experiments were repeated twice. Error bars represent standard deviations (SD). Significant changes (P < 0.05) are marked with an asterisk