Fig. 6.

miR-200b decreases the expression of CFTR mRNA during normoxia and hypoxia in primary normal human bronchial epithelial cells (NHBEC). a The levels of miR-200b after 12 h of hypoxia in NHBECs from 3 independent experiments (n = 6) are plotted normalized to RNU44 levels and expressed as a fold change over the normoxia control. b NHBEC cells were transfected with miR-200b mimic or antagomir and the mRNA levels were monitored in qRT-PCR experiments in normoxic conditions and after 12 h of hypoxia. CFTR mRNA levels from 2 independent experiments (n = 8) are plotted normalized to 18S rRNA levels and expressed as a fold change over the transfection control. c The corresponding changes of CFTR protein levels were monitored with SDS-PAGE and Western Blot analysis and normalized to the β-actin levels. Two individual samples (3 μg of total protein per lane) were tested for each treatment and the experiments were repeated twice. Error bars represent standard deviations (SD). Significant changes (P < 0.05) are marked with an asterisk