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. 2017 Nov 15;24:85. doi: 10.1186/s12929-017-0393-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effects of trypsin on ERK activation. Upper panel: Typical blot showing ERK phosphorylation in EA. hy926 cells treated with trypsin. EA. hy926 cells (1 × 10⁶/mL) were treated with 5 μg/mL of trypsin for the indicated duration, and p-ERK levels were determined through Western blotting as described and normalized to the total ERK levels. Lower panel: Normalization of the p-ERK and total ERK levels. Bar graphs represent means ± SEM from four independent experiments. *p < 0.05 compared with the control group