Fig. 2.

Percentage of embryos showing morphological defects at 72 hpf after microinjection with eight methylxanthines at 1-2 cell stage. For each drug, the percentage of embryos with mild (white bar) or severe (black bar) phenotype was assessed at each of the five tested concentrations. Embryos injected with dichloroaniline (DCA) were used as positive controls. At least 100 embryos were injected for each compound and the results are the mean ± SD of three independent experiments