Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 15;12:20. doi: 10.1186/s13064-017-0096-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 4 — Parallel bands of Müller glial scaffolding appear simultaneously with differentiating cones. (A-D) Live imaging of the retinal margin, with Red cone trβ2:tdTomato (red) and Müller glial gfap:EGFP (green) markers. Glial processes completely surround cone and rod photoreceptors, which can be visualized as round ‘holes’ in the GFP label at the level of the OLM. (A-A”) Maximum intensity z-projection. Red cones in the patterned lattice mosaic (white dots) and pre-column zone (magenta dots). (A”) Red cones (white dots) and non-red cones (open white dots) in the patterned lattice mosaic. (Also see Additional file 9: Movies S3 and Additional file 10: Movie S4.) (B-D) Single z-levels focused on the pre-column (B), and patterned (C, D) areas. (E-E”’) Ridge analysis applied to Müller glial profiles in flat-mount of Tg(gfap: EGFP) (green) retina immunolabeled for ZO1 (magenta). (Also see Additional file 7: Figure S4.) Arrows (E-E”) indicate the location of the first ordered column of cone photoreceptors that can be clearly discerned from cell outlines at the level of the OLM (E’). Parallel ridges of Müller glia extend for several iterations into the pre-column zone (E’-E”’). Scale bar: 5 μm (D”)