Figure 4.

Generation of the CRISPR/Cas9 Dnpep knockout mouse (A) Agarose gel showing genotyping of 10 CRISPR/cas9 offsping. An aliquot of the PCR reaction was digested with BspHI. Note the full‐length PCR fragment (lower band), digested products in samples labeled with a star, and absence of full‐length product in sample #24, indicating homozygosity. (B) Sequence of the two alleles of five mice showing mutations in red. The designed allele with stop codon (TAG) and BspHI restriction site is found in four of these mice. (C) Chromatogram of one heterozygous offspring showing germline transmission with one wild‐type sequence and one mutant sequence.