Table I.
Primary antibodies.
| Monoclonal antibody | Antigen | Dilution | Buffer |
|---|---|---|---|
| MA454a,c | MAGE-A1 | 1:200 | EDTA, 1 mM, pH 8.0 |
| 57Bb,c | MAGE-A4 | 1:4,000 | EDTA, 1 mM, pH 8.0 |
| 6C1a,c | MAGE-A 1, −2, −3, −4, −6, −10 and −12 | 1:20 | Citrate, 10 mM, pH 6.0 |
| CT7-33a | CT7 (MAGE-C1) | 1:32,000 | Citrate, 10 mM, pH 6.0 |
| #26d | GAGE | 1:80,000 | EDTA, 1 mM, pH 8.0 |
| E978a | CTAG1B | 1:3,200 | High pH retrieval solution |
a
Source: Ludwig Institute for Cancer Research, New York, NY, USA
b
Dr. Giulio Spagnoli, Department of Biomedicine, Basel, Switzerland
c
Included in the ‘Multi MAGE-A primary cocktail solution’ to detect the expression of MAGE-A family antigens
d
Transduction Labs, BD Biosciences, Franklin Lakes, NJ, USA. CT, cancer/testis antigen; MAGE, melanoma-associated antigen; CTAG1B, cancer/testis antigen 1B; GAGE, G antigen; EDTA, ethylenediaminetetraacetic acid.