Fig. 3.

The HCC vaccine promoted the generation of immune memory. PBMCs of BALB/c mice and C57BL/6 mice were isolated at the first week after the last immunization. The proportion of CD44⁺ CD62L⁺ cells in PBMCs from BALB/c mice (a & b) and C57BL/6 mice (c) was detected by FACS. d. The splenocytes of BALB/c mice were isolated at the first week after the last immunization, and an MTT assay was used to test the proliferation of the splenocytes treated or untreated (Control) with mitomycin-inactivated H22 cells for 5 days. PBS, mice immunized with PBS; Lipo, Scrm, and Decoy, mice immunized with Lipo, scrambled, or Decoy-ODN-treated HCC vaccine. Data are expressed as the mean ± SD, statistical significance was determined as *p < 0.05 and **p < 0.01 (n = 6)