. 2017 Oct 9;8(18):3648–3656. doi: 10.7150/jca.21783

Table 1.

Protocol of the experiment

Incubation of the drugs in lung cancer cell lines
1. Addition of chemotherapeutic agents alone for 2h 2. Addition of PDE inhibitors alone for 2h 3. Addition of combinations for 2h: chemotherapeutic agents (25μΜ) + PDE inhibitors (100μΜ) as follows: (sildenafil alone, roflumilast alone, theophylline alone, sildenafil + roflumilast, sildenafil + theophylline, roflumilast + theophylline, sildenafil + roflumilast + theophylline) 4. After 24h and 48h incubation, apoptosis was measured

Incubation of the drugs in lung cancer cell lines

1. Addition of chemotherapeutic agents alone for 2h
2. Addition of PDE inhibitors alone for 2h
3. Addition of combinations for 2h: chemotherapeutic agents (25μΜ) + PDE inhibitors (100μΜ) as follows: (sildenafil alone, roflumilast alone, theophylline alone, sildenafil + roflumilast, sildenafil + theophylline, roflumilast + theophylline, sildenafil + roflumilast + theophylline)
4. After 24h and 48h incubation, apoptosis was measured