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. 2017 Oct 17;8(18):3707–3717. doi: 10.7150/jca.20239

Figure 2.

Figure 2

Effect of STM on cell death and DNA fragmentation in HepG2 cells. (A) HepG2 cells were treated with indicated concentrations of STM in the presence or absence of 3 mM NAC for 24 h to determine cell death by live/dead cell assay using fluorescent probe calcein-AM and PI. Scale bar = 100 µM; (B) HepG2 cells were treated with indicated concentrations of STM in the presence or absence of 3 mM NAC for 24 h to examine nuclear morphological changes by Hoechst 33258 staining. Scale bar = 50 µM. 100 nuclei were counted from five different areas randomly for percentage of fragmented nuclei (apoptosis) in each group. Data are expressed as mean ± SD of three independent experiments. Columns not sharing the same superscript letters differ significantly (p<0.05).