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. 2017 Oct 17;8(18):3707–3717. doi: 10.7150/jca.20239

Figure 6.

Figure 6

Effect of STM on NF-кB activity and NF-кB regulators in HepG2 cells. HepG2 cells were treated with indicated concentrations of STM in the presence or absence of NAC for 24 h and proteins extracts (total/nuclear proteins) were prepared and analyzed by immunoblotting (A) to evaluate the effect of STM on NF-кB regulators and NF-кB activity (B) in the presence or absence of NAC (3mM). Data are expressed as Mean ± SD of three independent experiments. Columns not sharing the same superscript letter within the group differ significantly (p<0.05). (C) Effect of STM (50 µM) and IKK-16 (5 µM), an IкB kinase (IKK) inhibitor, on NF-кB activity after 24 h treatment. Nuclear proteins extracts were prepared and analyzed by immunoblotting. Data are expressed as Mean ± SD of three independent experiments. Columns not sharing the same superscript letter differ significantly (p<0.05). (D) Effect of STM on TNF-α-induced NF-кB activity. Cells were pretreated with TNF-α (50ng/ml) for 30 minutes followed by 24 h STM (100 µM) and IKK-16 (5 µM) treatment. Nuclear proteins extracts were prepared and analyzed by immunoblotting. Data are expressed as mean ± SD of three independent experiments. Columns not sharing the same superscript letters differ significantly (p<0.05).