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. 2017 Oct 17;8(18):3707–3717. doi: 10.7150/jca.20239

Figure 7.

Figure 7

Effect of STM on STAT3 activity and STAT3 regulators in HepG2 cells. HepG2 cells were treated with indicated concentrations of STM in the presence or absence of NAC for 24 h and proteins extracts were prepared and analyzed by immunoblotting to determine the effect of STM on expression of (A) STAT3, p-STAT3, (B) SHP2, (C) Src & p-Src. (D) HepG2 cells were treated with 100 µM of STM in the presence or absence of NAC (3 mM) for 24 h and proteins extracts were prepared and analyzed by immunoblotting to determine the effect of STM on expression of STAT3, p-STAT3 in the presence or absence of NAC. Data are expressed as Mean ± SD of three independent experiments. Columns not sharing the same superscript letter within the group differ significantly (p<0.05).