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. 2017 Oct 17;8(18):3803–3811. doi: 10.7150/jca.21228

Figure 2.

Figure 2

Studies on the molecular mechanisms of MALAT1-mediated miR-125b repression. A) Detection of Ago2 using western-blot, and detection of miR-125b using qRT-PCR. The error bars indicate the s.d. from five different experiments. **P < 0.01, relative to the beads group by paired, one-sided t-test. Uncropped blots were presented in Supplementary Figure 1. B) Ago2 siRNA or siRNA negative control was transfected into T24 cells. Then Ago2 protein expression was detected by western-blot. Uncropped blots were presented in Supplementary Figure 1. C) miR-125b expression was detected by qRT-PCR after co-transfection of CRISPR encoding plasmid and the siRNA. The error bars indicate the s.d. from five different experiments. NC, negative control. **P < 0.01, relative to the negative control group by paired, one-sided t-test.