Figure 3.
Effect of Huaier on the expression of epithelial and mesenchymal markers. (A) A heat map representing the mRNA expression levels of epithelial and mesenchymal markers. (B) Western blot assays of the expression levels of epithelial and mesenchymal markers after treatment with Huaier. (C) Confocal microscopic analysis of phenotypic markers, including E-cadherin, N-cadherin, and F-actin. The green signal represents staining for the corresponding proteins, red represents nuclear DNA staining by DAPI, and a white arrow indicates specifically stained cells.