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. Author manuscript; available in PMC: 2017 Nov 16.
Published in final edited form as: Nat Chem Biol. 2017 Mar 6;13(5):501–507. doi: 10.1038/nchembio.2317

Figure 1. Strand-specific total RNA-seq reveals global accumulation of nonpolyadenylated promoter-proximal RNA transcripts upon isoginkgetin (IsoG) treatment.

Figure 1

(a,b) Metaplots showing mean nonexonic read density across annotated promoter regions, aligned at transcription start sites (TSSs) and normalized to ERCC spike-in controls. (a) Strand-specific total RNA-seq in which ribosomal RNAs were depleted from total RNA by hybridization. (b) mRNA-seq in which poly(A)+ RNAs were purified from total RNA by oligo-dT hybridization. The traces represent an average of n = 2 replicates of 30 μM IsoG treatment of HeLa cells for 6 h. In these and subsequent TSS metaplots, only genes longer than the maximum x-axis value (here 10 kb) are included. S, sense; AS, antisense.