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. 2017 Dec;23(12):1850–1859. doi: 10.1261/rna.062182.117

FIGURE 4.

FIGURE 4.

SAXS data for MAL 123–218 and comparison of the ab initio models of MAL 123–218, NL4-3 PBS/TLE, and tRNALys3. (A) Plot of intensity vs. momentum transfer for MAL 123–218. The open black circles indicate every fifth data point from the experimental SAXS curve and the black line represents the back-calculated scattering curve of the ab initio envelope. (B) The pair distance distribution [P(r)] function showing a histogram of all interelectron distances in MAL 123–218. The open black circles represent the P(r) transformation of the experimental data, while the solid black line represents the back-calculated P(r) function of the ab initio envelope. (C) The ab initio envelopes of NL4-3 PBS/TLE and tRNALys3 are shown in black. The confirmed TLE and PBS regions of the NL4-3 PBS/TLE RNA are indicated (Jones et al. 2014). The ab initio envelope of MAL 123–218 is shown in red. The envelopes were superimposed using the SUPCOMB program (Konarev et al. 2006), and in the case of tRNALys3, followed by manual adjustments to optimize the fit.