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. 2017 Dec;23(12):1902–1926. doi: 10.1261/rna.061010.117

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© 2017 Kobyłecki et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 8. — Effect of mutations designed to change TtCutA specificity into PAP. (A) Length distribution for tails added by wild-type (WT) and mutant (PAP1-PAP6) TtCutA_tr3 variants to ss22-A₄ (left) and ss22-U₄ (right) oligoribonucleotide substrates. Numbers of analyzed sequences, obtained after 3′-RACE-seq high-throughput analysis for each protein variant, are indicated in parentheses. (B,C) Analysis of nucleotide composition for tails added by different TtCutA_tr3 variants to ss22-A₄ (panel B) and ss22-U₄ (panel C) RNA substrates. Results are presented as in Figure 6.